Mouse Monoclonal Antibody to NEFL
Item Information
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Description
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]
Product Overview
Entrez GenelD
4747
Aliases
NFL; NF-L; NF68; CMT1F; CMT2E; CMTDIG; PPP1R110
Clone#
2F8B3
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human NEFL (AA: 1-200) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1，Brain. 2020 Jan 1;143(1):47-54. 2，Lancet Neurol. 2019 Dec;18(12):1103-1111.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using NEFL mAb against human NEFL (AA: 1-200) recombinant protein. (Expected MW is 26.9 kDa)
Western Blot
Figure 3:Western blot analysis using NEFL mAb against HEK293-6e (1) and NEFL (AA: 1-200)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of Jurkat cells using NEFL mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 5:Flow cytometric analysis of SK-N-SH cells using NEFL mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded brain tissues using NEFL mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using NEFL mouse mAb with DAB staining.
Western Blot
Figure 8:Western blot analysis using NEFL mouse mAb against HEK293 (1) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.