NEFL Primary Antibody
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Description
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
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Western Blot

Figure 1: Western blot analysis using NEFL mouse mAb against Hela (1) and Jurkat (2) cell lysate.
Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using NEFL mouse mAb with DAB staining.
Immunohistochemical analysis

Figure 3: Immunohistochemical analysis of paraffin-embedded brain tissues using NEFL mouse mAb with DAB staining.
Immunofluorescence analysis

Figure 4: Immunofluorescence analysis of Hela cells using NEFL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric

Figure 5: Flow cytometric analysis of Jurkat cells using NEFL mouse mAb (green) and negative control (red).
Elisa

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.