NEFL Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesNFL; NF-L; NF68; CMT1F; CMT2E
ProductOrderN
Clone#2G10
Entrez GenelD4747
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human NEFL expressed in E. Coli.
MW62kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
422-543
Catalog#: 30313
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Images
Western Blot
Figure 1: Western blot analysis using NEFL mouse mAb against Hela (1) and Jurkat (2) cell lysate.
Figure 1: Western blot analysis using NEFL mouse mAb against Hela (1) and Jurkat (2) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using NEFL mouse mAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using NEFL mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded brain tissues using NEFL mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded brain tissues using NEFL mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 4: Immunofluorescence analysis of Hela cells using NEFL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 4: Immunofluorescence analysis of Hela cells using NEFL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 5: Flow cytometric analysis of Jurkat cells using NEFL mouse mAb (green) and negative control (red).
Figure 5: Flow cytometric analysis of Jurkat cells using NEFL mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.

References (references)
References (references)1. BMB Rep. 2008 Dec 31;41(12):868-74.
2. J Hum Genet. 2009 Feb;54(2):94-7.