Mouse Monoclonal Antibody to NEDD9

Item Information
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Description

The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Product Overview
Entrez GenelD
4739
Aliases
CAS2; CASL; HEF1; CAS-L; CASS2
Clone#
3F7A9
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human NEDD9 (AA: 82-398) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Biomolecules.2018 Dec 10;8(4):169.2.Biochem Biophys Res Commun.2019 Jan 29;509(1):201-208.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using NEDD9 mAb against human NEDD9 (AA: 82-398) recombinant protein. (Expected MW is 37.3 kDa)
Figure 2:Western blot analysis using NEDD9 mAb against human NEDD9 (AA: 82-398) recombinant protein. (Expected MW is 37.3 kDa)
Western Blot
Figure 4:Western blot analysis using NEDD9 mouse mAb against MCF-7 (1), Hela (2), C2C12 (3),and Hek293 (4) cell lysate.
Figure 4:Western blot analysis using NEDD9 mouse mAb against MCF-7 (1), Hela (2), C2C12 (3),and Hek293 (4) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using NEDD9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of Hela cells using NEDD9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 6:Flow cytometric analysis of HepG2 cells using NEDD9 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of HepG2 cells using NEDD9 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 7:Flow cytometric analysis of Jurkat cells using NEDD9 mouse mAb (green) and negative control (red).
Figure 7:Flow cytometric analysis of Jurkat cells using NEDD9 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 8:Flow cytometric analysis of K562 cells using NEDD9 mouse mAb (green) and negative control (red).
Figure 8:Flow cytometric analysis of K562 cells using NEDD9 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.