NEDD8 Primary Antibody

Item Information
Catalog #
Size
Price
Description

Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.Tissue specificity: Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.

Product Overview
Entrez GenelD
4738
Aliases
NEDD-8
Clone#
1A7
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human NEDD8 expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Cell. 2009 Jul 23;138(2):389-403.
2. Biochem Biophys Res Commun. 2009 Apr 10;381(3):443-7.
Product Image
Western Blot
Figure 1: Western blot analysis using NEDD8 mAb against human NEDD8 (AA: 1-81) recombinant protein. (Expected MW is 40 kDa)
Figure 1: Western blot analysis using NEDD8 mAb against human NEDD8 (AA: 1-81) recombinant protein. (Expected MW is 40 kDa)
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using NEDD8 mouse mAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using NEDD8 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of Hela cells using NEDD8 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 3: Immunofluorescence analysis of Hela cells using NEDD8 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 4: Flow cytometric analysis of Hela cells using NEDD8 mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of Hela cells using NEDD8 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.