NDC80

Item Information

Catalog #

Size

Price

Description

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation.

Product Overview

Entrez GenelD

10403

Aliases

HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80

Clone#

2B1G6

Host / Isotype

Mouse / Mouse IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human NDC80 (AA: 443-642) expressed in mammalian.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4℃; -20℃ for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1,Mol Biol Cell . 2020 Jul 1;31(14):1453-1473.
2,J Biol Chem . 2019 Jan 11;294(2):576-592.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using NDC80 mAb against human NDC80 (AA: 443-642) recombinant protein. (Expected MW is 26 kDa)

Western Blot

Figure 3:Western blot analysis using NDC80 mouse mAb against Hela (1), HepG2 (2), Jurkat (3), K562 (4) and A431 (5) cell lysate.

Immunohistochemical analysis

Figure 4:Immunofluorescence analysis of Hela cells using NDC80 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Immunofluorescence analysis