This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation.
Purified antibody in PBS with 0.05% sodium azide
4℃; -20℃ for long term storage
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using NDC80 mAb against human NDC80 (AA: 443-642) recombinant protein. (Expected MW is 26 kDa)
Figure 3:Western blot analysis using NDC80 mouse mAb against Hela (1), HepG2 (2), Jurkat (3), K562 (4) and A431 (5) cell lysate.
Figure 4:Immunofluorescence analysis of Hela cells using NDC80 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Flow cytometric analysis of Hela cells using NDC80 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.