NDC80

Item Information
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Price
Description

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation.

Product Overview
Entrez GenelD
10403
Aliases
HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80
Clone#
1D11B8
Host / Isotype
Mouse / Mouse IgG2a
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human NDC80 (AA: 443-642) expressed in mammalian cells.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Mol Biol Cell . 2020 Jul 1;31(14):1453-1473.
2,J Biol Chem . 2019 Jan 11;294(2):576-592.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using NDC80 mAb against human NDC80 (AA: 443-642) recombinant protein. (Expected MW is 26 kDa)
Figure 2:Western blot analysis using NDC80 mAb against human NDC80 (AA: 443-642) recombinant protein. (Expected MW is 26 kDa)
Western Blot
Figure 3:Western blot analysis using NDC80 mouse mAb against Hela (1), HepG2 (2), Jurkat (3)and K562 (4) cell lysate.
Figure 3:Western blot analysis using NDC80 mouse mAb against Hela (1), HepG2 (2), Jurkat (3)and K562 (4) cell lysate.
Immunohistochemical analysis
Figure 4:Immunofluorescence analysis of Hela cells using NDC80 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of Hela cells using NDC80 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of Hela cells using NDC80 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Hela cells using NDC80 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded mammary cancer tissues using NDC80 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded mammary cancer tissues using NDC80 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using NDC80 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using NDC80 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.