NCAM1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants.
Product Overview
Entrez GenelD
4684
Aliases
CD56; NCAM; MSK39
Clone#
5B1G11
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human NCAM1 (AA: extra(568-708)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200-1/1000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1?Clin Chim Acta. 2019 Aug;495:374-376.2?Blood. 2019 May 23;133(21):2305-2319.
Product Image
ELISA
![Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-elisa_31893ea_222.jpg?v=1666704820)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
![Figure 2: Western blot analysis using NCAM1 mAb against NCAM1 (AA: extra(568-708)) recombinant protein. (Expected MW is 40.1 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-wb_31893rwb_223.jpg?v=1666704820)
Figure 2: Western blot analysis using NCAM1 mAb against NCAM1 (AA: extra(568-708)) recombinant protein. (Expected MW is 40.1 kDa)
WESTERN BLOT
![Figure 3: Western blot analysis using NCAM1 mAb against HEK293 (1) and NCAM1 (AA: extra(568-708))-hIgGFc transfected HEK293 (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-wb_31893twb_224.jpg?v=1666704820)
Figure 3: Western blot analysis using NCAM1 mAb against HEK293 (1) and NCAM1 (AA: extra(568-708))-hIgGFc transfected HEK293 (2) cell lysate.
WESTERN BLOT
![Figure 4: Western blot analysis using NCAM1 mouse mAb against SH-SY5Y (1), COS-7 (2), and NIH3T3 (3) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-wb_31893lwb_225.jpg?v=1666704820)
Figure 4: Western blot analysis using NCAM1 mouse mAb against SH-SY5Y (1), COS-7 (2), and NIH3T3 (3) cell lysate.
FLOW CYTOMETRY
![Figure 5: Flow cytometric analysis of Hela cells using NCAM1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-fcm_31893fcm_226.jpg?v=1666704820)
Figure 5: Flow cytometric analysis of Hela cells using NCAM1 mouse mAb (green) and negative control (red).
IMMUNOHISTOCHEMISTRY
![Figure 6: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using NCAM1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-icc_31893ihc_227.jpg?v=1666704820)
Figure 6: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using NCAM1 mouse mAb with DAB staining.
IMMUNOHISTOCHEMISTRY
![Figure 7: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using NCAM1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-icc_31893ihc-2_228.jpg?v=1666704820)
Figure 7: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using NCAM1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.