NCAM1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants.
Product Overview
Entrez GenelD
4684
Aliases
CD56; NCAM; MSK39
Clone#
2C5E7
Host / Isotype
Mouse / Mouse IgG2a
Immunogen
Purified recombinant fragment of human NCAM1 (AA: extra(568-708)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1?Clin Chim Acta. 2019 Aug;495:374-376.2?Blood. 2019 May 23;133(21):2305-2319.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using NCAM1 mAb against human NCAM1 (AA: extra(568-708)) recombinant protein. (Expected MW is 40.1 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using NCAM1 mAb against HEK293-6e (1) and NCAM1 (AA: extra(568-708))-hIgGFc transfected HEK293-6e (2) cell lysate.
WESTERN BLOT
Figure 4: Western blot analysis using NCAM1 mouse mAb against SH-SY5Y (1), and Hela (2) cell lysate.
FLOW CYTOMETRY
Figure 5: Flow cytometric analysis of Hela cells using NCAM1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.