NANOG Primary Antibody

Item Information

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Price

Description

NANOG: Nanog homeobox. Entrez Protein NP_079141. Nanog is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human Nanog protein shares 52% overall amino acid identity with the mouse protein and 85% identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst. High levels of human Nanog expression were detected by Northern analysis in the undifferentiated N-Tera embryonal carcinoma cell line.

Product Overview

Entrez GenelD

79923

Aliases

NANOG

Clone#

1E6C4

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of NANOG (aa20-166) expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Genes Cells. 2006 Sep;11(9):1115-23.
2. Mol Biol Cell. 2007 May;18(5):1543-53.

Product Image

Western Blot

Figure 1: Western blot analysis using NANOG mouse mAb against NTERA-2 cell lysate (2).

Immunofluorescence analysis

Figure 2: Confocal Immunofluorescence analysis of NTERA-2 cells (left) and HeLa cells (right) using Nanog mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.

For Research Use Only. Not for use in diagnostic procedures.