NAA10 Primary Antibody

Item Information

Catalog #

Size

Price

Description

N-alpha-acetylation is among the most common post-translational protein modifications in eukaryotic cells. This process involves the transfer of an acetyl group from acetyl-coenzyme A to the alpha-amino group on a nascent polypeptide and is essential for normal cell function. This gene encodes an N-terminal acetyltransferase that functions as the catalytic subunit of the major amino-terminal acetyltransferase A complex. Mutations in this gene are the cause of Ogden syndrome. Alternate splicing results in multiple transcript variants.

Product Overview

Entrez GenelD

8260

Aliases

TE2; ARD1; NATD; ARD1A; ARD1P; OGDNS; DXS707; MCOPS1

Clone#

3G3B9

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Mouse, Monkey

Immunogen

Purified recombinant fragment of human NAA10 (AA: 111-235) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Gene. 2015 Aug 10;567(2):103-31.
2.PLoS One. 2014 Aug 18;9(8):e105185.

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using NAA10 mAb against human NAA10 (AA: 111-235) recombinant protein. (Expected MW is 47.2 kDa)

Western Blot

Figure 3:Western blot analysis using NAA10 mAb against HEK293 (1) and NAA10 (AA: 111-235)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using NAA10 mouse mAb against COS7 (1), HEK293 (2), HL-60 (3), MCF-7 (4), Hela (5), NIH/3T3 (6), and C2C12 (7) cell lysate.

Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using NAA10 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric