MYOD1
Item Information
Catalog #
Size
Price
Description
This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.
Product Overview
Entrez GenelD
4654
Aliases
PUM; MYF3; MYOD; bHLHc1; MYODRIF
Clone#
3B4C3
Host / Isotype
Mouse / Mouse IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human MYOD1 (AA: 221-320) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/50 - 1/200
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Hum Pathol. 2020 May;99:75-79.
2.Sci Rep. 2021 Feb 4;11(1):3021.
2.Sci Rep. 2021 Feb 4;11(1):3021.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using MYOD1 mAb against human MYOD1 (AA: 221-320) recombinant protein. (Expected MW is 48 kDa)
Western Blot
Figure 3:Western blot analysis using MYOD1 mAb against HEK293-6e (1) and MYOD1 (AA: 221-320)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunohistochemical analysis
Figure 4:Immunofluorescence analysis of Hela cells using MYOD1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of Hela cells using MYOD1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.