MYOD1
Item Information
Catalog #
Size
Price
Description
This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Image
Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot

Figure 2:Western blot analysis using MYOD1 mAb against human MYOD1 (AA: 221-320) recombinant protein. (Expected MW is 48 kDa)
Western Blot

Figure 3:Western blot analysis using MYOD1 mAb against HEK293-6e (1) and MYOD1 (AA: 221-320)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunohistochemical analysis

Figure 4:Immunofluorescence analysis of Hela cells using MYOD1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis

Figure 5:Flow cytometric analysis of Hela cells using MYOD1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.