Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using MYOD1 mAb against human MYOD1 (AA: 221-320) recombinant protein. (Expected MW is 48 kDa)
Figure 3:Western blot analysis using MYOD1 mAb against HEK293-6e (1) and MYOD1 (AA: 221-320)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 4:Immunofluorescence analysis of Hela cells using MYOD1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Flow cytometric analysis of Hela cells using MYOD1 mouse mAb (green) and negative control (red).