MYOD1 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.

Product Overview

Entrez GenelD

4654

Aliases

PUM; MYF3; MYOD; bHLHc1; MYODRIF

Clone#

3B4C3

Host / Isotype

Mouse / Mouse IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human MYOD1 (AA: 221-320) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/50 - 1/200

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Hum Pathol. 2020 May;99:75-79.
2.Sci Rep. 2021 Feb 4;11(1):3021.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using MYOD1 mAb against human MYOD1 (AA: 221-320) recombinant protein. (Expected MW is 48 kDa)

Western Blot

Figure 3:Western blot analysis using MYOD1 mAb against HEK293-6e (1) and MYOD1 (AA: 221-320)-hIgGFc transfected HEK293-6e (2) cell lysate.

Immunohistochemical analysis

Figure 4:Immunofluorescence analysis of Hela cells using MYOD1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Immunofluorescence analysis