MYCN Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
4613
Aliases
NMYC; ODED; MODED; N-myc; bHLHe37
Clone#
2A4E9
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human MYCN (AA: 337-464) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200-1/1000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1.Pediatr Blood Cancer. 2018 Oct;65(10):e27257. 2.Proc Natl Acad Sci U S A. 2018 May 8;115(19):4969-4974.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using MYCN mAb against human MYCN (AA: 337-464) recombinant protein. (Expected MW is 17.7 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using MYCN mAb against HEK293 (1) and MYCN (AA: 337-464)-hIgGFc transfected HEK293 (2) cell lysate.
FLOW CYTOMETRY
Figure 4: Flow cytometric analysis of Hela cells using MYCN mouse mAb (green) and negative control (red).
IMMUNOHISTOCHEMISTRY
Figure 5: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using MYCN mouse mAb with DAB staining.
IMMUNOHISTOCHEMISTRY
Figure 6: Immunohistochemical analysis of paraffin-embedded cerebellum tissues using MYCN mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.