MuRF1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that localizes to the Z-line and M-line lattices of myofibrils. This protein plays an important role in the atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy chain proteins, myosin light chain, myosin binding protein, and for muscle-type creatine kinase.
Product Overview
Entrez GenelD
84676
Aliases
TRIM63; IRF; SMRZ; MURF2; RNF28
Clone#
7B8E1
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Synthesized peptide of human MuRF1 (AA: 293-304).
Formulation
Purified antibody in PBS with 0.05% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Liver Int. 2013 May;33(5):714-21. doi: 10.1111/liv.12128. Epub 2013 Feb 24.
2. Cell Biochem Biophys. 2011 Jun;60(1-2):113-8.
2. Cell Biochem Biophys. 2011 Jun;60(1-2):113-8.
Product Image
Immunofluorescence analysis
Figure 1:Immunofluorescence analysis of Hela cells using MuRF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 2: Flow cytometric analysis of Hela cells using MuRF1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MuRF1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded muscle tissues using MuRF1 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.