MUC5B Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a member of the mucin family of proteins, which are highly glycosylated macromolecular components of mucus secretions. This family member is the major gel-forming mucin in mucus. It is a major contributor to the lubricating and viscoelastic properties of whole saliva, normal lung mucus and cervical mucus. This gene has been found to be up-regulated in some human diseases, including sinus mucosa of chronic rhinosinusitis (CRS), CRS with nasal polyposis, chronic obstructive pulmonary disease (COPD) and H. pylori-associated gastric disease, and it may be involved in the pathogenesis of these diseases.
Product Overview
Entrez GenelD
727897
Aliases
MG1; MUC5; MUC9; MUC-5B
Clone#
7F5A1
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human MUC5B (AA: 26-166) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Am J Respir Cell Mol Biol. 2017 Jul;57(1):91-99.
2.Int J Oncol. 2016 May;48(5):2113-23.
2.Int J Oncol. 2016 May;48(5):2113-23.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using MUC5B mAb against human MUC5B (AA: 26-166) recombinant protein. (Expected MW is 41.4 kDa)
Western Blot
Figure 3:Western blot analysis using MUC5B mAb against HEK293 (1) and MUC5B (AA: 26-166)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HL-60 cells using MUC5B mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded rectum tissues using MUC5B mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.