Mouse Monoclonal Antibody to MUC12

Item Information

Catalog #

Size

Price

Description

This gene encodes an integral membrane glycoprotein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces and have been implicated in epithelial renewal and differentiation. These glycoproteins also play a role in intracellular signaling. This protein is expressed on the apical membrane surface of epithelial cells that line the mucosal surfaces of many different tissues including the colon, pancreas, prostate, and uterus. The expression of this gene is downregulated in colorectal cancer tissue.

Product Overview

Entrez GenelD

10071

Aliases

MUC11; MUC-11;

Clone#

2C6E1

Host / Isotype

Mouse / IgG2b

Immunogen

Purified recombinant fragment of human MUC12 (AA: extra 371-592) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4℃; -20℃ for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/50 - 1/200

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Mol Biochem Parasitol. 2019 Jan;227:19-24 2.Int J Cancer. 2010 Nov 15;127(10):2292-9.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using MUC12 mAb against human MUC12 (AA: extra 371-592) recombinant protein. (Expected MW is 53.3 kDa)

Immunofluorescence analysis

Figure 3:Immunofluorescence analysis of Hela cells using MUC12 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis