mTOR Primary Antibody
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | FRAP; FRAP1; FRAP2; RAFT1; RAPT1 |
| ProductOrder | M |
| Clone# | 5E4D8 |
| Entrez GenelD | 2475 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Host | Mouse |
| Isotype | IgG2a |
| Immunogen | Purified recombinant fragment of human mTOR (AA: 2311-2529) expressed in E. Coli. |
| MW | 289kDa |
| Shipping Information | This product will ship in a box containing blue ice at a temperature of 4°C. Learn More |
| Species Reactivity | Human |
| Application | |
|---|---|
| ELISA | 1/10000 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| 2311-2529 | |
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using mTOR mAb against human mTOR (AA: 2311-2529) recombinant protein. (Expected MW is 50.8 kDa)
Western Blot
Figure 3:Western blot analysis using mTOR mAb against HEK293 (1) and mTOR (AA: 2311-2529)-hIgGFc transfected HEK293 (2) cell lysate.