MTDH
Item Information
Catalog #
Size
Price
Description
MTDH (Metadherin) is a Protein Coding gene. Diseases associated with MTDH include Tongue Carcinoma and Gallbladder Adenocarcinoma. Among its related pathways are Gastric Cancer Network 2 and Validated targets of C-MYC transcriptional activation. Gene Ontology (GO) annotations related to this gene include double-stranded RNA binding. An important paralog of this gene is MAMSTR.
Product Overview
Entrez GenelD
92140
Aliases
3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3
Clone#
1F3A3
Host / Isotype
Mouse / Mouse IgG1
Species Reactivity
Human, Monkey
Immunogen
Purified recombinant fragment of human MTDH (AA: 200-450) expressed in mammalian.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/50 - 1/200
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Cell Death Dis. 2020 Apr 6;11(4):221.
2,Cancer Res. 2021 Feb 15;81(4):1014-1025.
2,Cancer Res. 2021 Feb 15;81(4):1014-1025.
Product Image
Elisa
![Figure1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32328-1ea.jpg?v=1669029539)
Figure1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using MTDH mAb against human MTDH (AA: 200-450) recombinant protein. (Expected MW is 57.8 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32328rwb.jpg?v=1669029538)
Figure 2:Western blot analysis using MTDH mAb against human MTDH (AA: 200-450) recombinant protein. (Expected MW is 57.8 kDa)
Western Blot
![Figure 3:Western blot analysis using MTDH mouse mAb against MCF-7 (1), T47D (2), Jurkat (3) ,K562 (4), Hela(5), PC-3(6), HepG2(7), A431(8) and COS7 (9) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32328lwb.jpg?v=1669029538)
Figure 3:Western blot analysis using MTDH mouse mAb against MCF-7 (1), T47D (2), Jurkat (3) ,K562 (4), Hela(5), PC-3(6), HepG2(7), A431(8) and COS7 (9) cell lysate.
Immunohistochemical analysis
![Figure 4:Immunofluorescence analysis of Hela cells using MTDH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32328icc.jpg?v=1669029539)
Figure 4:Immunofluorescence analysis of Hela cells using MTDH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
![Figure 5:Flow cytometric analysis of Hela cells using MTDH mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32328fcm.jpg?v=1669029539)
Figure 5:Flow cytometric analysis of Hela cells using MTDH mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 6:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using MTDH mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32328ihc.jpg?v=1669029539)
Figure 6:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using MTDH mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 7:Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using MTDH mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32328-1ihc.jpg?v=1669029539)
Figure 7:Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using MTDH mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.