MTDH
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | 3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3 |
| Clone# | 1F3A3 |
| Entrez GenelD | 92140 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Host | Mouse |
| Isotype | Mouse IgG1 |
| Immunogen | Purified recombinant fragment of human MTDH (AA: 200-450) expressed in mammalian. |
| MW | 75KDa |
| Species Reactivity | Human, Monkey |
| Application | |
|---|---|
| ELISA | 1/10000 |
| ICC (Immunocytochemistry) | 1/50 - 1/200 |
| IHC_P(Immunohistochemistry) | 1/200 - 1/1000 |
| FCM (Flow Cytometry) | 1/200 - 1/400 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| 200-450 | |
Elisa
Figure1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using MTDH mAb against human MTDH (AA: 200-450) recombinant protein. (Expected MW is 57.8 kDa)
Western Blot
Figure 3:Western blot analysis using MTDH mouse mAb against MCF-7 (1), T47D (2), Jurkat (3) ,K562 (4), Hela(5), PC-3(6), HepG2(7), A431(8) and COS7 (9) cell lysate.
Immunohistochemical analysis
Figure 4:Immunofluorescence analysis of Hela cells using MTDH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of Hela cells using MTDH mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using MTDH mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using MTDH mouse mAb with DAB staining.