MTDH Primary Antibody

Item Information

Catalog #

Size

Price

Description

MTDH (Metadherin) is a Protein Coding gene. Diseases associated with MTDH include Tongue Carcinoma and Gallbladder Adenocarcinoma. Among its related pathways are Gastric Cancer Network 2 and Validated targets of C-MYC transcriptional activation. Gene Ontology (GO) annotations related to this gene include double-stranded RNA binding. An important paralog of this gene is MAMSTR.

Product Overview

Entrez GenelD

92140

Aliases

3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3

Clone#

1F3A3

Host / Isotype

Mouse / Mouse IgG1

Species Reactivity

Human, Monkey

Immunogen

Purified recombinant fragment of human MTDH (AA: 200-450) expressed in mammalian.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/50 - 1/200

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1,Cell Death Dis. 2020 Apr 6;11(4):221.
2,Cancer Res. 2021 Feb 15;81(4):1014-1025.

Product Image

Elisa

Figure1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using MTDH mAb against human MTDH (AA: 200-450) recombinant protein. (Expected MW is 57.8 kDa)

Western Blot

Figure 3:Western blot analysis using MTDH mouse mAb against MCF-7 (1), T47D (2), Jurkat (3) ,K562 (4), Hela(5), PC-3(6), HepG2(7), A431(8) and COS7 (9) cell lysate.

Immunohistochemical analysis

Figure 4:Immunofluorescence analysis of Hela cells using MTDH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Immunofluorescence analysis