MTDH

Item Information
Catalog #Size/ConcentrationPrice
Specification
Aliases3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3
Clone#1F3A3
Entrez GenelD92140
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeMouse IgG1
ImmunogenPurified recombinant fragment of human MTDH (AA: 200-450) expressed in mammalian.
MW75KDa
Species ReactivityHuman, Monkey
Application
ELISA1/10000
ICC (Immunocytochemistry)1/50 - 1/200
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
200-450
Catalog#: 32328
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Images
Elisa
Figure1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using MTDH mAb against human MTDH (AA: 200-450) recombinant protein. (Expected MW is 57.8 kDa)
Figure 2:Western blot analysis using MTDH mAb against human MTDH (AA: 200-450) recombinant protein. (Expected MW is 57.8 kDa)
Western Blot
Figure 3:Western blot analysis using MTDH mouse mAb against MCF-7 (1), T47D (2), Jurkat (3) ,K562 (4), Hela(5), PC-3(6), HepG2(7), A431(8) and COS7 (9) cell lysate.
Figure 3:Western blot analysis using MTDH mouse mAb against MCF-7 (1), T47D (2), Jurkat (3) ,K562 (4), Hela(5), PC-3(6), HepG2(7), A431(8) and COS7 (9) cell lysate.
Immunohistochemical analysis
Figure 4:Immunofluorescence analysis of Hela cells using MTDH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of Hela cells using MTDH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of Hela cells using MTDH mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Hela cells using MTDH mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using MTDH mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using MTDH mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using MTDH mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using MTDH mouse mAb with DAB staining.
Product Overview
Description

MTDH (Metadherin) is a Protein Coding gene. Diseases associated with MTDH include Tongue Carcinoma and Gallbladder Adenocarcinoma. Among its related pathways are Gastric Cancer Network 2 and Validated targets of C-MYC transcriptional activation. Gene Ontology (GO) annotations related to this gene include double-stranded RNA binding. An important paralog of this gene is MAMSTR.

References (references)
References (references)1,Cell Death Dis. 2020 Apr 6;11(4):221.
2,Cancer Res. 2021 Feb 15;81(4):1014-1025.