MTA1 Primary Antibody

Item Information
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Price
Description

This gene encodes a protein that was identified in a screen for genes expressed in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this gene has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues. The role it plays in metastasis is unclear. It was initially thought to be the 70kD component of a nucleosome remodeling deacetylase complex, NuRD, but it is more likely that this component is a different but very similar protein. These two proteins are so closely related, though, that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. The profile and activity of this gene product suggest that it is involved in regulating transcription and that this may be accomplished by chromatin remodeling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]

Product Overview
Entrez GenelD
9112
Clone#
6B1E9
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human MTA1 (AA: 541-715) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.PLoS One. 2017 Jun 1;12(6):e0176431.
2.Oncol Rep. 2015 Feb;33(2):885-92.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using MTA1 mAb against human MTA1 (AA: 541-715) recombinant protein. (Expected MW is 45.3 kDa)
Figure 2:Western blot analysis using MTA1 mAb against human MTA1 (AA: 541-715) recombinant protein. (Expected MW is 45.3 kDa)
Western Blot
Figure 3:Western blot analysis using MTA1 mAb against HEK293 (1) and MTA1 (AA: 541-715)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using MTA1 mAb against HEK293 (1) and MTA1 (AA: 541-715)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using MTA1 mouse mAb against SW480 (1), T47D (2), MCF-7 (3), and COS7 (4) cell lysate.
Figure 4:Western blot analysis using MTA1 mouse mAb against SW480 (1), T47D (2), MCF-7 (3), and COS7 (4) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of Hela cells using MTA1 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Hela cells using MTA1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.