MSLN Primary Antibody
Item Information
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Price
Description
This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
Product Overview
Entrez GenelD
10232
Aliases
MPF; SMRP
Clone#
7E6A6
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human MSLN (AA: 296-606) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4?; -20? for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
1.Tumour Biol. 2018 Oct;40(10):1010428318804937. 2.Mol Cancer. 2017 Mar 14;16(1):63.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using MSLN mAb against human MSLN (AA: 296-606) recombinant protein. (Expected MW is 30.5 kDa)
Western Blot
Figure 3:Western blot analysis using MSLN mAb against HEK293 (1) and MSLN (AA: 296-606)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using MSLN mouse mAb against PC-3 (1), SK-MES-1 (2), and CHO3D10 (3) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.