Mouse Monoclonal Antibody to MSH2
Item Information
Catalog #
Size
Price
Description
This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
4436
Aliases
FCC1; COCA1; HNPCC; LCFS2; hMSH2; HNPCC1; MMRCS2
Clone#
1H8C1
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human MSH2 (AA: 2-151) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Clin Epigenetics. 2019 Oct 30;11(1):153.2,Genes Chromosomes Cancer. 2020 Feb;59(2):111-118.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/51b8372874224311064eea15b6908f7313e6c5d3f24c468c986c88873d95a068.jpg?v=1666705497)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using MSH2 mAb against human MSH2 (AA: 2-151) recombinant protein. (Expected MW is 19.6kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/13723399490f38bc427697e0f8d31a518e1f0003978d6fdc90bc9df4e012b9fc.jpg?v=1666705497)
Figure 2:Western blot analysis using MSH2 mAb against human MSH2 (AA: 2-151) recombinant protein. (Expected MW is 19.6kDa)
Western Blot
![Figure 3:Western blot analysis using MSH2 mAb against HEK293-6e (1) and MSH2 (AA: 2-151)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/4120f048aaa0bae86ba10f18122e42f2019cf4e4acb65ed1a50431e7a81d67c7.jpg?v=1666705497)
Figure 3:Western blot analysis using MSH2 mAb against HEK293-6e (1) and MSH2 (AA: 2-151)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
![Figure 4:Western blot analysis using MSH2 mouse mAb against MCF-7 (1), A431 (2),K562 (3),Hela (4), Raji (5),and A549 (6) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/462cb2f1e24a9cd2cae62a61ca96c9050d9e00ca7aece8e876b154b791a6a923.jpg?v=1666705497)
Figure 4:Western blot analysis using MSH2 mouse mAb against MCF-7 (1), A431 (2),K562 (3),Hela (4), Raji (5),and A549 (6) cell lysate.
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of Hela cells using MSH2 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/cfb2839b1bdb1259065ee480951a3f0fcf1bbc7eb02a34c0d55512d0aaeee8ec.jpg?v=1666705497)
Figure 5:Flow cytometric analysis of Hela cells using MSH2 mouse mAb (green) and negative control (red).
Flow cytometric analysis
![Figure 6:Flow cytometric analysis of K562 cells using MSH2 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/fcecb70915edc50951443f18e98474c08256640fbc1a66145082974358687eab.jpg?v=1666705497)
Figure 6:Flow cytometric analysis of K562 cells using MSH2 mouse mAb (green) and negative control (red).
Flow cytometric analysis
![Figure 7:Flow cytometric analysis of Raji cells using MSH2 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/6ebc62ecc05718950f31c397947a990848a927be9b9fc55f83e6fd14565ca6dc.jpg?v=1666705497)
Figure 7:Flow cytometric analysis of Raji cells using MSH2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 8:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using MSH2 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/c006235bc978e0c305dc81eb139e7c8e1d5b0f9cfd330bcff0221b963ed23324.jpg?v=1666705497)
Figure 8:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using MSH2 mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 9:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using MSH2 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/42d9744636d91bc1b0343b1896c6804fe70b5ae5d22d3073eb0cd3c3b298976d.jpg?v=1666705497)
Figure 9:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using MSH2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.