MSH2 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
4436
Aliases
FCC1; COCA1; HNPCC; LCFS2; hMSH2; HNPCC1
Clone#
2E2C7
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human MSH2 (AA: 442-586) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4?; -20? for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Anticancer Res. 2018 May;38(5):2841-2848. 2.Clin Cancer Res. 2017 Nov 15;23(22):6863-6874.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using MSH2 mAb against human MSH2 (AA: 442-586) recombinant protein. (Expected MW is 19.6 kDa)
Western Blot
Figure 3:Western blot analysis using MSH2 mAb against HEK293 (1) and MSH2 (AA: 442-586)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using MSH2 mouse mAb against Hela (1), NIH/3T3 (2), A549 (3), and A431 (4) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using MSH2 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using MSH2 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Flow cytometric
Figure 7:Flow cytometric analysis of Hela cells using MSH2 mouse mAb (green) and negative control (red).
Immunohistochemical Analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using MSH2 mouse mAb with DAB staining.
Immunohistochemical Analysis
Figure 9:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MSH2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.