MRE11
Item Information
Catalog #
Size
Price
Description
This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
Product Overview
Entrez GenelD
4361
Aliases
ATLD; HNGS1; MRE11A; MRE11B
Clone#
4D2B6
Host / Isotype
Mouse / Mouse IgG1
Species Reactivity
Human, Rat
Immunogen
Purified recombinant fragment of human MRE11 (AA: 182-582) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cancer Lett. 2021 Aug 28;514:1-11.
2.Diagn Pathol. 2019 Jun 21;14(1):60.
2.Diagn Pathol. 2019 Jun 21;14(1):60.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using MRE11 mAb against human MRE11 (AA: 182-582) recombinant protein. (Expected MW is 49.2 kDa)
Western Blot
Figure 3:Western blot analysis using MRE11 mAb against HEK293-6e (1) and MRE11 (AA: 182-582)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis
Figure 4:Flow cytometric analysis of Hela cells using MRE11 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of K562 cells using MRE11 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using MRE11 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using MRE11 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MRE11 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 9:Immunohistochemical analysis of paraffin-embedded rat brain tissues using MRE11 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 10:Immunohistochemical analysis of paraffin-embedded rat cerebellum tissues using MRE11 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 11:Immunohistochemical analysis of paraffin-embedded rat kidney tissues using MRE11 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.