MPL Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesMPLV; TPOR; C-MPL; CD110
ProductOrderM
Clone#1H2
Entrez GenelD4352
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG2b
ImmunogenPurified recombinant fragment of human MPL expressed in E. Coli.
MW71.2kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
307-362
Catalog#: 30309
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Images
Western Blot
Figure 1: Western blot analysis using MPL mAb against human MPL (AA: 307-362) recombinant protein. (Expected MW is 32.2 kDa)
Figure 1: Western blot analysis using MPL mAb against human MPL (AA: 307-362) recombinant protein. (Expected MW is 32.2 kDa)
Flow cytometric
Figure 2: Flow cytometric analysis of MOLT4 cells using MPL mouse mAb (green) and negative control (red).
Figure 2: Flow cytometric analysis of MOLT4 cells using MPL mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.

References (references)
References (references)Cancer Res. 2009 Apr 15;69(8):3681-8.
J Biol Chem. 2009 May 1;284(18):11781-91.