MMP2 Primary Antibody

Item Information
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Size
Price
Description
This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.
Product Overview
Entrez GenelD
4313
Aliases
CLG4; MONA; CLG4A; MMP-2; TBE-1; MMP-II
Clone#
2B11E6
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human MMP2 (AA: 9-140) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Reprod Biol Endocrinol. 2015 Sep 4;13:102.
2.PLoS One. 2015 Mar 27;10(3):e0121404.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using MMP2 mAb against human MMP2 (AA: 9-140) recombinant protein. (Expected MW is 42.2 kDa)
Figure 2:Western blot analysis using MMP2 mAb against human MMP2 (AA: 9-140) recombinant protein. (Expected MW is 42.2 kDa)
Western Blot
Figure 3:Western blot analysis using MMP2 mAb against HEK293 (1) and MMP2 (AA: 9-140)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using MMP2 mAb against HEK293 (1) and MMP2 (AA: 9-140)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Hela cells using MMP2 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of Hela cells using MMP2 mouse mAb (green) and negative control (red).
Western Blot
Figure 5:Western blot analysis using MMP2 mouse mAb against MCF-7 (1), Raw264.7 (2), HUVEC (3), and T47D (4) cell lysate.
Figure 5:Western blot analysis using MMP2 mouse mAb against MCF-7 (1), Raw264.7 (2), HUVEC (3), and T47D (4) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.