MLH1 Primary Antibody

Item Information
Catalog #
Size
Price
Description

The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC).

Product Overview
Entrez GenelD
4292
Aliases
FCC2; COCA2; HNPCC; hMLH1; HNPCC2
Clone#
5A3F6
Host / Isotype
Mouse / Mouse IgG2b
Immunogen
Purified recombinant fragment of human MLH1 (AA: 381-483) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4?; -20? for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cancer Med. 2018 Feb;7(2):433-444. 2.Genes Chromosomes Cancer. 2017 Sep;56(9):681-690.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using MLH1 mAb against human MLH1 (AA: 381-483) recombinant protein. (Expected MW is 25.1 kDa)
Figure 2:Western blot analysis using MLH1 mAb against human MLH1 (AA: 381-483) recombinant protein. (Expected MW is 25.1 kDa)
Western Blot
Figure 3:Western blot analysis using MLH1 mAb against HEK293 (1) and MLH1 (AA: 381-483)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using MLH1 mAb against HEK293 (1) and MLH1 (AA: 381-483)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometric
Figure 4:Flow cytometric analysis of Hela cells using MLH1 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of Hela cells using MLH1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.