Mouse Monoclonal Antibody to MKI67
Item Information
Catalog #
Size
Price
Description
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.
Product Overview
Entrez GenelD
4288
Aliases
KIA; MIB-; MIB-1; PPP1R105
Clone#
2E1F1
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human MKI67 (AA: 1160-1493) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Histopathology. 2019 Dec;75(6):853-864. 2.Pancreas. 2019 Jul;48(6):795-798.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/1afaf0ee5a00b7fc705a44310684291fc6bf46161d24367ca98207730c989866.jpg?v=1666705243)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using MKI67 mAb against human MKI67 (AA: 1160-1493) recombinant protein. (Expected MW is 39 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/4d10f1170bfa67874074454f54a95b8a7983c4727e25ddd606ff0755084739d7.jpg?v=1666705243)
Figure 2:Western blot analysis using MKI67 mAb against human MKI67 (AA: 1160-1493) recombinant protein. (Expected MW is 39 kDa)
Flow cytometric analysis
![Figure 3:Flow cytometric analysis of THP-1 cells using MKI67 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/250a7db5e46b4c8fe262284a897dcc24381a452744e9ea9f5cef6065eb30fc96.jpg?v=1666705243)
Figure 3:Flow cytometric analysis of THP-1 cells using MKI67 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 4:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using MKI67 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/3177ff2bf0aa38e3d7e68a5d973a767ff22ea795ce0d7572bacec79d02e9317e.jpg?v=1666705244)
Figure 4:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using MKI67 mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 5:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using MKI67 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/9cdbae511ae610e807ee069a87da6573110138b1e5f461eb6c9ba478f9c0c998.jpg?v=1666705244)
Figure 5:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using MKI67 mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using MKI67 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/5b35e819fddb6730505769c4b47790a0808335ac75e7c9f767a854a31700f363.jpg?v=1666705244)
Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using MKI67 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.