Mouse Monoclonal Antibody to MKI67

Item Information
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Size
Price
Description

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.

Product Overview
Entrez GenelD
4288
Aliases
KIA; MIB-; MIB-1; PPP1R105
Clone#
7B12B1
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human MKI67 (AA: 1160-1493) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Histopathology. 2019 Dec;75(6):853-864. 2.Pancreas. 2019 Jul;48(6):795-798.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using MKI67 mAb against human MKI67 (AA: 1160-1493) recombinant protein. (Expected MW is 39 kDa)
Figure 2:Western blot analysis using MKI67 mAb against human MKI67 (AA: 1160-1493) recombinant protein. (Expected MW is 39 kDa)
Flow cytometric analysis
Figure 3:Flow cytometric analysis of THP-1 cells using MKI67 mouse mAb (green) and negative control (red).
Figure 3:Flow cytometric analysis of THP-1 cells using MKI67 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 4:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using MKI67 mouse mAb with DAB staining.
Figure 4:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using MKI67 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MKI67 mouse mAb with DAB staining.
Figure 5:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MKI67 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.