Mouse Monoclonal Antibody to MKI67
Item Information
Catalog #
Size
Price
Description
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.
Product Overview
Entrez GenelD
4288
Aliases
KIA; MIB-; MIB-1; PPP1R105
Clone#
7B12B1
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human MKI67 (AA: 1160-1493) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Histopathology. 2019 Dec;75(6):853-864. 2.Pancreas. 2019 Jul;48(6):795-798.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/9f2205d099b1edaa146ed45ca4c5d8c74be036cf9ff9bf87e96f0a512dcae900.jpg?v=1666705235)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using MKI67 mAb against human MKI67 (AA: 1160-1493) recombinant protein. (Expected MW is 39 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/b0d2e412b00704c6116af34e3a0bea5dce466e66df840a91adc4d1a322e5c405.jpg?v=1666705235)
Figure 2:Western blot analysis using MKI67 mAb against human MKI67 (AA: 1160-1493) recombinant protein. (Expected MW is 39 kDa)
Flow cytometric analysis
![Figure 3:Flow cytometric analysis of THP-1 cells using MKI67 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/a838c6b1687cd1ebddc6c199040e5d77287408b04532c4b87854e315f257477d.jpg?v=1666705235)
Figure 3:Flow cytometric analysis of THP-1 cells using MKI67 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 4:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using MKI67 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/537d8329fbf4007c522132c3533fdb3a2bcbcfc47e106e2b5d07c42a104e9ab2.jpg?v=1666705235)
Figure 4:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using MKI67 mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 5:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MKI67 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/5d305f938d760f262d5bdae68e91116ae94f77f2cd1ef3bb982fb65a00f4acc9.jpg?v=1666705235)
Figure 5:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MKI67 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.