Mouse Monoclonal Antibody to MICB
Item Information
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Description
This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules; however, it does not associate with beta-2-microglobulin or bind peptides. Alternative splicing results in multiple transcript variants.
Product Overview
Entrez GenelD
4277
Aliases
PERB11.2
Clone#
1B2F11
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human MICB (AA: extra 23-309) expressed in HEK293-6e cells supernatant.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.HLA. 2018 Oct;92(4):224-230. 2.BMC Infect Dis. 2015 Jan 23;15:25.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using MICB mAb against human MICB (AA: extra 23-309) recombinant protein. (Expected MW is 63.2 kDa)
Flow cytometric analysis
Figure 3:Flow cytometric analysis of THP-1 cells using MICB mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.