MGMT Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesEC 2.1.1.63
ProductOrderM
Clone#1A11F8
Entrez GenelD4255
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human MGMT (AA: 32-210) expressed in E. Coli.
MW21.6kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
32-210
Catalog#: 30837
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using MGMT mAb against human MGMT (AA: 32-210) recombinant protein. (Expected MW is 45.2 kDa)
Figure 2:Western blot analysis using MGMT mAb against human MGMT (AA: 32-210) recombinant protein. (Expected MW is 45.2 kDa)
Western Blot
Figure 3:Western blot analysis using MGMT mAb against HEK293 (1) and MGMT (AA: 32-210)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using MGMT mAb against HEK293 (1) and MGMT (AA: 32-210)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using MGMT mouse mAb against MCF-7 (1), Jurkat (2), HepG2 (3), and PC-3 (4) cell lysate.
Figure 4:Western blot analysis using MGMT mouse mAb against MCF-7 (1), Jurkat (2), HepG2 (3), and PC-3 (4) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using MGMT mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of Hela cells using MGMT mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of Hela cells using MGMT mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of Hela cells using MGMT mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MGMT mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MGMT mouse mAb with DAB staining.
Product Overview
DescriptionMGMT (O-6-methylguanine-DNA methyltransferase) is a protein-coding gene. Diseases associated with MGMT include oligoastrocytoma, and spinal cord astrocytoma. GO annotations related to this gene include DNA-methyltransferase activity and calcium ion binding.
References (references)
References (references)1.Neuropathology. 2014 Jun;34(3):268-76.
2.Clin Cancer Res. 2013 Apr 15;19(8):2265-72.