MGMT Primary Antibody
Item Information
Catalog #
Size
Price
Description
MGMT involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA.Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated
Product Overview
Entrez GenelD
4255
Aliases
N
Clone#
1A11A8
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human MGMT (AA: 32-210) expressed in E. Coli.
Formulation
Purified antibody from tissue culture in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
Folia Neuropathol. 2013;51(4):275-82.
Fam Cancer. 2013 Sep;12(3):449-58.
Fam Cancer. 2013 Sep;12(3):449-58.
Product Image
Western Blot
Figure 1: Figure 3:Western blot analysis using MGMT mAb against HEK293 (1) and MGMT (AA: 32-210)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 2:Western blot analysis using MGMT mAb against human MGMT (AA: 32-210) recombinant protein. (Expected MW is 45.2 kDa)
Elisa
Figure 2:Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.