Mouse Monoclonal Antibody to MERTK
Item Information
Catalog #
Size
Price
Description
This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP).
Product Overview
Entrez GenelD
10461
Aliases
MER; RP38; c-Eyk; c-mer; Tyro12
Clone#
3D11G8
Host / Isotype
Mouse / IgG2b
Immunogen
Purified recombinant fragment of human MERTK (AA:extra(21-220)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,PLoS One. 2019 Dec 5;14(12):e0225051. 2,Can J Ophthalmol. 2019 Feb;54(1):40-50.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using MERTK mAb against human MERTK (AA:extra(21-220)) recombinant protein. (Expected MW is 25kDa)
Western Blot
Figure 3:Western blot analysis using MERTK mAb against HEK293-6e (1) and MERTK (AA:extra(21-220))-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using MERTK mouse mAb against HepG2 (1), and PANC-1 (2) cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of bel-7402 cells using MERTK mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 6:Flow cytometric analysis of HepG2 cells using MERTK mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 7:Flow cytometric analysis of U937 cells using MERTK mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 8:Immunofluorescence analysis of Hela cells using MERTK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.