MELK Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesHPK38
ProductOrderM
Clone#2G2
Entrez GenelD9833
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG2a
ImmunogenSynthesized peptide of human MELK(AA: 637-651:C-VYKRLVEDILSSCKV).
MW74.6kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
Sequence
637-651:C-VYKRLVEDILSSCKV
Catalog#: 30374
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Images
Immunohistochemical analysis
Figure 1: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using MELK mouse mAb with DAB staining.
Figure 1: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using MELK mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MELK mouse mAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MELK mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of HepG2 cells using MELK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 3: Immunofluorescence analysis of HepG2 cells using MELK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 4: Flow cytometric analysis of MCF-7 cells using MELK mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of MCF-7 cells using MELK mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

Maternal embryonic leucine-zipper kinase (MELK) is a key regulator of survival of stemlike GBM cells in vitro. MELK expression is increased in breast cancer tissue and this increase is also associated with poor patient survival, as predicted for a candidate oncogene.

References (references)
References (references)1. Neuro Oncol. 2011 Jun;13(6):622-34.
2. Breast Cancer Res. 2009;11(4):R60.