MELK Primary Antibody

Item Information

Catalog #

Size

Price

30374

100ul

$435.0

Description

Maternal embryonic leucine-zipper kinase (MELK) is a key regulator of survival of stemlike GBM cells in vitro. MELK expression is increased in breast cancer tissue and this increase is also associated with poor patient survival, as predicted for a candidate oncogene.

Product Overview

Entrez GenelD

9833

Aliases

HPK38

Clone#

2G2

Host / Isotype

Mouse / IgG2a

Species Reactivity

Human

Immunogen

Synthesized peptide of human MELK(AA: 637-651:C-VYKRLVEDILSSCKV).

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. Neuro Oncol. 2011 Jun;13(6):622-34.
2. Breast Cancer Res. 2009;11(4):R60.

Product Image

Immunohistochemical analysis

Figure 1: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using MELK mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MELK mouse mAb with DAB staining.

Immunofluorescence analysis

Figure 3: Immunofluorescence analysis of HepG2 cells using MELK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric