MDM2 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.
Product Overview
Entrez GenelD
4193
Aliases
HDMX; LSKB; hdm2; ACTFS
Clone#
3C1E3
Host / Isotype
Mouse / Mouse IgG2b
Immunogen
Purified recombinant fragment of human MDM2 (AA: 26-169) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200-1/1000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1.Cancer Genomics Proteomics. 2018 Sep-Oct;15(5):405-411. 2.Int J Mol Sci. 2017 Oct 23;18(10). pii: E2216.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using MDM2 mAb against human MDM2 (AA: 26-169) recombinant protein. (Expected MW is *** kDa)
WESTERN BLOT
Figure 3: Western blot analysis using MDM2 mAb against HEK293 (1) and MDM2 (AA: 26-169)-hIgGFc transfected HEK293 (2) cell lysate.
FLOW CYTOMETRY
Figure 4: Flow cytometric analysis of Hela cells using MDM2 mouse mAb (green) and negative control (red).
IMMUNOHISTOCHEMISTRY
Figure 5: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using MDM2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.