MCM2 Primary Antibody
|Aliases||BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN; KIAA0030; MGC10606|
|Formulation||Ascitic fluid containing 0.03% sodium azide. |
|Immunogen||Purified recombinant fragment of human MCM2 expressed in E. Coli. |
|Shipping Information||This product will ship in a box containing blue ice at a temperature of 4°C. Learn More|
|ICC (Immunocytochemistry)||1/200 - 1/1000|
|IHC_P(Immunohistochemistry)||1/200 - 1/1000|
|FCM (Flow Cytometry)||1/200 - 1/400|
|WB (Western Blot)||1/500 - 1/2000|
Contact us to order
Figure 1: Western blot analysis using MCM2 mAb against human MCM2 (AA: 16-232) recombinant protein. (Expected MW is 50.4 kDa)
Figure 2: Western blot analysis using MCM2 mouse mAb against MCF-7 (1), Hela (2), Jurkat (3), K562 (4), HEK293 (5) and HEPG2 (6) cell lysate.
Figure 3: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using MCM2 mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using MCM2 mouse mAb with DAB staining.
Figure 5: Immunofluorescence analysis of Hela cells using MCM2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 6: Flow cytometric analysis of Jurkat cells using MCM2 mouse mAb (green) and negative control (red).
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
|References (references)||1. Mol Cell. 2009 Jul 31;35(2):206-16. |
2. J Cutan Pathol. 2009 Oct;36(10):1121-2.