MCAM Primary Antibody
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | CD146; MUC18 |
| ProductOrder | M |
| Clone# | 6C3E6 |
| Entrez GenelD | 4162 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Purified recombinant fragment of human MCAM (AA: 84-189) expressed in E. Coli. |
| MW | 71.6kDa |
| Shipping Information | This product will ship in a box containing blue ice at a temperature of 4°C. Learn More |
| Species Reactivity | Human |
| Application | |
|---|---|
| ELISA | 1/10000 |
| IHC_P(Immunohistochemistry) | 1/200 - 1/1000 |
| FCM (Flow Cytometry) | 1/200 - 1/400 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| 84-189 | |
Western Blot
Figure 1: Western blot analysis using MCAM mAb against human MCAM recombinant protein. (Expected MW is 37.7 kDa)
Western Blot
Figure 2: Western blot analysis using MCAM mAb against HEK293 (1) and MCAM (AA: 84-189)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using MCAM mouse mAb against HUVE-12 (1), EVC-304 (2), HELA (3) and MCF-7 (4) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of MCF-7 cells using MCAM mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using MCAM mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using MCAM mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);