MCAM Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
Product Overview
Entrez GenelD
4162
Aliases
CD146; MUC18
Clone#
6C3E6
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human MCAM (AA: 84-189) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Cancer Lett. 2013 Apr 28;330(2):150-62.
2. J Biol Chem. 2013 Jan 25;288(4):2571-9.
2. J Biol Chem. 2013 Jan 25;288(4):2571-9.
Product Image
Western Blot
Figure 1: Western blot analysis using MCAM mAb against human MCAM recombinant protein. (Expected MW is 37.7 kDa)
Western Blot
Figure 2: Western blot analysis using MCAM mAb against HEK293 (1) and MCAM (AA: 84-189)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using MCAM mouse mAb against HUVE-12 (1), EVC-304 (2), HELA (3) and MCF-7 (4) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of MCF-7 cells using MCAM mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using MCAM mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using MCAM mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.