MCAM Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesCD146; MUC18
ProductOrderM
Clone#6C3E6
Entrez GenelD4162
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human MCAM (AA: 84-189) expressed in E. Coli.
MW71.6kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
84-189
Catalog#: 30505
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Images
Western Blot
Figure 1: Western blot analysis using MCAM mAb against human MCAM recombinant protein. (Expected MW is 37.7 kDa)
Figure 1: Western blot analysis using MCAM mAb against human MCAM recombinant protein. (Expected MW is 37.7 kDa)
Western Blot
Figure 2: Western blot analysis using MCAM mAb against HEK293 (1) and MCAM (AA: 84-189)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using MCAM mAb against HEK293 (1) and MCAM (AA: 84-189)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using MCAM mouse mAb against HUVE-12 (1), EVC-304 (2), HELA (3) and MCF-7 (4) cell lysate.
Figure 3: Western blot analysis using MCAM mouse mAb against HUVE-12 (1), EVC-304 (2), HELA (3) and MCF-7 (4) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of MCF-7 cells using MCAM mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of MCF-7 cells using MCAM mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using MCAM mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using MCAM mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using MCAM mouse mAb with DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using MCAM mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
DescriptionThe protein encoded by this gene plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

References (references)
References (references)1. Cancer Lett. 2013 Apr 28;330(2):150-62.
2. J Biol Chem. 2013 Jan 25;288(4):2571-9.