MB Primary Antibody

Item Information
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Description

This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported.

Product Overview
Entrez GenelD
4151
Aliases
PVALB; myoglobgin
Clone#
3B6D5
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human MB (AA: 2-154) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4?; -20? for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Stroke Cerebrovasc Dis. 2016 Jul;25(7):1582-1589. 2.PLoS One. 2015 Nov 11;10(11):e0142662.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using MB mAb against human MB (AA: 2-154) recombinant protein. (Expected MW is 20 kDa)
Figure 2:Western blot analysis using MB mAb against human MB (AA: 2-154) recombinant protein. (Expected MW is 20 kDa)
Western Blot
Figure 3:Western blot analysis using MB mAb against HEK293 (1) and MB (AA: 2-154)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using MB mAb against HEK293 (1) and MB (AA: 2-154)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometric
Figure 4:Flow cytometric analysis of Hela cells using MB mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of Hela cells using MB mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.