MB Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported.

Product Overview

Entrez GenelD

4151

Aliases

PVALB

Clone#

6B9D2

Host / Isotype

Mouse / IgG2a

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human MB (AA: 34-126) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Lung Cancer. 2011 Dec;74(3):411-8.
2. Br J Cancer. 2010 Jun 8;102(12):1736-45.

Product Image

Western Blot

Figure 1: Western blot analysis using MB mAb against human MB (AA: 34-126) recombinant protein. (Expected MW is 36.3 kDa)

Western Blot

Figure 2: Western blot analysis using MB mAb against HEK293 (1) and MB (AA: 34-126)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 3: Immunofluorescence analysis of Hela cells using MB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Immunohistochemical analysis