MAP4K1 Primary Antibody
Item Information
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Size
Price
Description
MAP4K1 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1) is a Protein Coding gene. Among its related pathways are Angiopoietin Like Protein 8 Regulatory Pathway and B cell receptor signaling pathway (KEGG). Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is MAP4K3.
Product Overview
Entrez GenelD
11184
Aliases
HPK1
Clone#
5A10F1
Host / Isotype
Mouse / Mouse IgG1
Species Reactivity
Human, Mouse, Monkey, Rat
Immunogen
Purified recombinant fragment of human MAP4K1 (AA:278-477) expressed in mammalian.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Acta Crystallogr F Struct Biol Commun. 2021 Jan 1;77(Pt 1):22-28.
2.EBioMedicine. 2021 Jul;69:103441.
2.EBioMedicine. 2021 Jul;69:103441.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using MAP4K1 mAb against human MAP4K1 (AA: 278-477) recombinant protein. (Expected MW is 52.3 kDa)
Western Blot
Figure 3:Western blot analysis using MAP4K1 mouse mAb against Jurkat (1), A549 (2), mouse kidney (3),COS7 (4),C6 (5) and NIH/3T3 (6) cell lysate.
Immunofluorescence analysis
Figure 4:Flow cytometric analysis of Jurkat cells using MAP4K1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using MAP4K1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using MAP4K1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.