MAP3K2 Primary Antibody

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase preferentially activates other kinases involved in the MAP kinase signaling pathway. This kinase has been shown to directly phosphorylate and activate Ikappa B kinases, and thus plays a role in NF-kappa B signaling pathway. This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a regulated signaling process.

Product Overview

Entrez GenelD

10746

Aliases

MEKK2; MEKK2B

Clone#

4B4

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human MAP3K2 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Clin Cancer Res. 2009 Sep 1;15(17):5541-51.
2. J Biol Chem. 2009 May 15;284(20):13533-41.

Product Image

Western Blot

Figure 1: Western blot analysis using MAP3K2 mAb against human MAP3K2 (AA: 148-359) recombinant protein. (Expected MW is 49.2 kDa)

Western Blot

Figure 2: Western blot analysis using MAP3K2 mAb against HEK293 (1) and MAP3K2(AA: 148-359)--hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 3: Immunofluorescence analysis of 3T3-L1 cells using MAP3K2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Elisa