MAP3K2 Primary Antibody
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | MEKK2; MEKK2B |
| ProductOrder | M |
| Clone# | 4B4 |
| Entrez GenelD | 10746 |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Purified recombinant fragment of human MAP3K2 expressed in E. Coli. |
| MW | 70kDa |
| Shipping Information | This product will ship in a box containing blue ice at a temperature of 4°C. Learn More |
| Species Reactivity | Human |
| Application | |
|---|---|
| ELISA | 1/10000 |
| ICC (Immunocytochemistry) | 1/200 - 1/1000 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| 148-359 | |
Western Blot
Figure 1: Western blot analysis using MAP3K2 mAb against human MAP3K2 (AA: 148-359) recombinant protein. (Expected MW is 49.2 kDa)
Western Blot
Figure 2: Western blot analysis using MAP3K2 mAb against HEK293 (1) and MAP3K2(AA: 148-359)--hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of 3T3-L1 cells using MAP3K2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);