MAP2K4 Primary Antibody
Item Information
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Price
Description
This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.Tissue specificity: Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues .
Product Overview
Entrez GenelD
6416
Aliases
JNKK; MEK4; MKK4; SEK1; JNKK1; SERK1; MAPKK4; PRKMK4; MAP2K4
Clone#
5H4
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human MAP2K4 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. J Biol Chem. 2009 Jan 2;284(1):685-95.
2. J Immunol. 2008 Sep 1;181(5):3252-8.
2. J Immunol. 2008 Sep 1;181(5):3252-8.
Product Image
Western Blot
Figure 1: Western blot analysis using MAP2K4 mouse mAb against HepG2 (1), K562 (2), and HEK293 (3) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded muscle tissues using MAP2K4 mouse mAb with DAB staining.
Flow cytometric
Figure 3: Flow cytometric analysis of K562 cells using MAP2K4 mouse mAb (green) and negative control (purple).
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
For Research Use Only. Not for use in diagnostic procedures.