Mouse Monoclonal Antibody to MAGE-3
Item Information
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Price
Description
This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. [provided by RefSeq, Jul 2008]
Product Overview
Entrez GenelD
4102
Aliases
HIP8; HYPD; CT1.3; MAGE3; MAGEA6
Clone#
4D2E7
Host / Isotype
Mouse / IgG2a
Immunogen
Purified recombinant fragment of human MAGE-3 (AA: FLWGPRALVC) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Biomed Pharmacother. 2020 Feb;122:109710. 2,J Clin Oncol. 2017 Oct 10;35(29):3322-3329.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Immunofluorescence analysis
Figure 2:Immunofluorescence analysis of Hela cells using MAGE-3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 3:Flow cytometric analysis of Hela cells using MAGE-3 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.