Mouse Monoclonal Antibody to M6PR
Item Information
Catalog #
Size
Price
Description
This gene encodes a member of the P-type lectin family. P-type lectins play a critical role in lysosome function through the specific transport of mannose-6-phosphate-containing acid hydrolases from the Golgi complex to lysosomes. The encoded protein functions as a homodimer and requires divalent cations for ligand binding. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. A pseudogene of this gene is located on the long arm of chromosome X.
Product Overview
Entrez GenelD
4074
Aliases
SMPR; MPR46; CD-MPR; MPR 46; MPR-46; CD-M6PR
Clone#
3A10B2
Host / Isotype
Mouse / IgG2b
Immunogen
Purified recombinant fragment of human M6PR (AA: 124-277) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cell Mol Immunol. 2018 Nov;15(11):986-988. 2.Biochemistry. 2010 Jan 12;49(1):236-46.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/0dc350672878c82be6eb39d1d02d4dc4a2d3b6a15dee22d50bfb64eda08f9d83.jpg?v=1666705947)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using M6PR mAb against human M6PR (AA: 124-277) recombinant protein. (Expected MW is 43 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/7d62821c7058956abc82cde5fa4b35c167e84d1d27bc7d5393bd4f5c15cc1c74.jpg?v=1666705947)
Figure 2:Western blot analysis using M6PR mAb against human M6PR (AA: 124-277) recombinant protein. (Expected MW is 43 kDa)
Western Blot
![Figure 3:Western blot analysis using M6PR mAb against HEK293-6e (1) and M6PR (AA: 124-277)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/e4ea782c3a34f65813f74d6c1b0301caca2f287d3fabf3184e3b69da7f392341.jpg?v=1666705947)
Figure 3:Western blot analysis using M6PR mAb against HEK293-6e (1) and M6PR (AA: 124-277)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
![Figure 4:Western blot analysis using M6PR mouse mAb against mouse brain (1) and HepG2 (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/d5188e025ce210e52a15c07e1f0d81c72ba121508195af30f827812d51a096da.jpg?v=1666705947)
Figure 4:Western blot analysis using M6PR mouse mAb against mouse brain (1) and HepG2 (2) cell lysate.
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of Jurkat cells using M6PR mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/7601fcbe2bc2e01697a95e8080c6ae9749e33870a7b9c924eef9aef83f02db64.jpg?v=1666705947)
Figure 5:Flow cytometric analysis of Jurkat cells using M6PR mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 6:Immunohistochemical analysis of paraffin-embedded human brain tissues using M6PR mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/4d27277abdfb490f34969bd978364eee107b705e0099ebab7bc98110b3fe48b8.jpg?v=1666705947)
Figure 6:Immunohistochemical analysis of paraffin-embedded human brain tissues using M6PR mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.