Mouse Monoclonal Antibody to M6PR
Item Information
Catalog #
Size
Price
Description
This gene encodes a member of the P-type lectin family. P-type lectins play a critical role in lysosome function through the specific transport of mannose-6-phosphate-containing acid hydrolases from the Golgi complex to lysosomes. The encoded protein functions as a homodimer and requires divalent cations for ligand binding. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. A pseudogene of this gene is located on the long arm of chromosome X.
Product Overview
Entrez GenelD
4074
Aliases
SMPR; MPR46; CD-MPR; MPR 46; MPR-46; CD-M6PR
Clone#
1B11C1
Host / Isotype
Mouse / IgG2b
Immunogen
Purified recombinant fragment of human M6PR (AA: 124-277) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cell Mol Immunol. 2018 Nov;15(11):986-988. 2.Biochemistry. 2010 Jan 12;49(1):236-46.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/43a29b1147506a9a8fb6c3bc7e2bd3746b12bbabdc3734c4d27dbb176cb9077a.jpg?v=1666705942)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using M6PR mAb against human M6PR (AA: 124-277) recombinant protein. (Expected MW is 43 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/20d1d5b0eae18a3e35fd494590f901eb305eef9decb4cb68a45f8643c5e41171.jpg?v=1666705942)
Figure 2:Western blot analysis using M6PR mAb against human M6PR (AA: 124-277) recombinant protein. (Expected MW is 43 kDa)
Western Blot
![Figure 3:Western blot analysis using M6PR mAb against HEK293-6e (1) and M6PR (AA: 124-277)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/5a9b2b265f16f00b5b83a916a98719ac8ead97d4262dec6965134e8a1709d33d.jpg?v=1666705942)
Figure 3:Western blot analysis using M6PR mAb against HEK293-6e (1) and M6PR (AA: 124-277)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
![Figure 4:Western blot analysis using M6PR mouse mAb against mouse brain (1) and HepG2 (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/8dc39817334c84eb8daca759a8936aa04e4a226c3d15c6cf4b06b8243d6e0a5e.jpg?v=1666705942)
Figure 4:Western blot analysis using M6PR mouse mAb against mouse brain (1) and HepG2 (2) cell lysate.
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of THP-1 cells using M6PR mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/9ba0c269e7adb0d4995654163e768d0d5a2efc3b3eb533476e46fb0d5a4ec28b.jpg?v=1666705942)
Figure 5:Flow cytometric analysis of THP-1 cells using M6PR mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 6:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using M6PR mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/624aaa3f08cf9bdd38ed9d83836653ec56cd73041826781271544ff5f1b6c7ee.jpg?v=1666705943)
Figure 6:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using M6PR mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 7:Immunohistochemical analysis of paraffin-embedded human brain tissues using M6PR mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/212ecab3449e5ec1d0a4af1e7f04c99a31d52c9bce3dde7bb6162dad8c4a6941.jpg?v=1666705943)
Figure 7:Immunohistochemical analysis of paraffin-embedded human brain tissues using M6PR mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.