LRRK2
Item Information
Catalog #
Size
Price
Description
This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8.
Product Overview
Entrez GenelD
120892
Aliases
PARK8; RIPK7; ROCO2; AURA17; DARDARIN
Clone#
2A4B12
Host / Isotype
Mouse / Mouse IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human LRRK2 (AA: 300-530) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Neurodegener Dis. 2020;20(2-3):65-72.
2.Cells. 2020 Oct 31;9(11):2394.
2.Cells. 2020 Oct 31;9(11):2394.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using LRRK2 mAb against human LRRK2 (AA: 300-530) recombinant protein. (Expected MW is 29.3 kDa)
Western Blot
Figure 3:Western blot analysis using LRRK2 mAb against HEK293-6e (1) and LRRK2 (AA: 300-530)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis
Figure 4:Flow cytometric analysis of Hela cells using LRRK2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded brain tissues using LRRK2 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using LRRK2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.