Mouse Monoclonal Antibody to LMNB1

Item Information

Catalog #

Size

Price

Description

This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants.

Product Overview

Entrez GenelD

4001

Aliases

LMN；ADLD；LMN2; LMNB；MCPH26

Clone#

2B11D5

Host / Isotype

Mouse / IgG1

Immunogen

Purified recombinant fragment of human LMNB1 (AA: 413-583) expressed in HEK293-6e cells supernatant.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4℃; -20℃ for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.J Immunol. 2019 Mar 1;202(5):1428-1440. 2.Am J Hum Genet. 2020 Oct 1;107(4):753-762.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using LMNB1 mAb against human LMNB1 (AA: 413-583) recombinant protein. (Expected MW is 49.4 kDa)

Western Blot

Figure 3:Western blot analysis using LMNB1 mouse mAb against Hela (1), Hek293 (2), K562 (3), SH-SY5Y (4), and PC-3 (5) cell lysate.

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of Hela cells using LMNB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis