Mouse Monoclonal Antibody to LMNB1

Item Information
Catalog #
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Price
Description

This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants.

Product Overview
Entrez GenelD
4001
Aliases
LMN;ADLD;LMN2; LMNB;MCPH26
Clone#
2B11D5
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human LMNB1 (AA: 413-583) expressed in HEK293-6e cells supernatant.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Immunol. 2019 Mar 1;202(5):1428-1440. 2.Am J Hum Genet. 2020 Oct 1;107(4):753-762.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using LMNB1 mAb against human LMNB1 (AA: 413-583) recombinant protein. (Expected MW is 49.4 kDa)
Figure 2:Western blot analysis using LMNB1 mAb against human LMNB1 (AA: 413-583) recombinant protein. (Expected MW is 49.4 kDa)
Western Blot
Figure 3:Western blot analysis using LMNB1 mouse mAb against Hela (1), Hek293 (2), K562 (3), SH-SY5Y (4), and PC-3 (5) cell lysate.
Figure 3:Western blot analysis using LMNB1 mouse mAb against Hela (1), Hek293 (2), K562 (3), SH-SY5Y (4), and PC-3 (5) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using LMNB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of Hela cells using LMNB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 5:Flow cytometric analysis of Hela cells using LMNB1 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Hela cells using LMNB1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using LMNB1 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using LMNB1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded pancreas tissues using LMNB1 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded pancreas tissues using LMNB1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.