Mouse Monoclonal Antibody to LMNB1
Item Information
Catalog #
Size
Price
Description
This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants.
Product Overview
Entrez GenelD
4001
Aliases
LMN;ADLD;LMN2; LMNB;MCPH26
Clone#
2A11A2
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human LMNB1 (AA: 413-583) expressed in HEK293-6e cells supernatant.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Immunol. 2019 Mar 1;202(5):1428-1440. 2.Am J Hum Genet. 2020 Oct 1;107(4):753-762.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/c0f21062f37ccac8d2ac55c2631039ee79b2ed5d848bb52dcc1510caaa05500f.jpg?v=1666705792)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using LMNB1 mAb against human LMNB1 (AA: 413-583) recombinant protein. (Expected MW is 49.4 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/ddab902a3f84e7b307d09220235e6b14b973ccc967d97c23759a30da54b6209e.jpg?v=1666705792)
Figure 2:Western blot analysis using LMNB1 mAb against human LMNB1 (AA: 413-583) recombinant protein. (Expected MW is 49.4 kDa)
Western Blot
![Figure 3:Western blot analysis using LMNB1 mouse mAb against Hela (1), HEK2933 (2), K562 (3), SH-SY5Y (4), and PC-3 (5) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/bce03f4e4dd561b0bce37c6f4d76df9e768b8c2bbc2f10d282a1467416f823a9.jpg?v=1666705792)
Figure 3:Western blot analysis using LMNB1 mouse mAb against Hela (1), HEK2933 (2), K562 (3), SH-SY5Y (4), and PC-3 (5) cell lysate.
Immunofluorescence analysis
![Figure 3:Immunofluorescence analysis of Hela cells using LMNB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/1b67b97729e52fbc9d4a25070516c441d360cb2f2430c0de46bf52301e62cd29.jpg?v=1666705792)
Figure 3:Immunofluorescence analysis of Hela cells using LMNB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of Hela cells using LMNB1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30a738e53a33d4a8d838ed0346f98c2eb0f04e163b82bd6bd20d3dc03dbef05a.jpg?v=1666705792)
Figure 5:Flow cytometric analysis of Hela cells using LMNB1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 6:Immunohistochemical analysis of paraffin-embedded colon tissues using LMNB1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/d84a66e80464fc5a5b5553f6581a4198cdee5bd52c6c81bc3b3c2014740d0d01.jpg?v=1666705792)
Figure 6:Immunohistochemical analysis of paraffin-embedded colon tissues using LMNB1 mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using LMNB1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/9d732669f7e92e77fd64fe41b1b2e8c03b2c8030d45bc99a502be2112cffa0a0.jpg?v=1666705792)
Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using LMNB1 mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 8:Immunohistochemical analysis of paraffin-embedded brain tumors tissues using LMNB1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/e25b60c6ca3ca67a611b6710676640091700e61e3f49ffa987c8d2744cc51b4c.jpg?v=1666705792)
Figure 8:Immunohistochemical analysis of paraffin-embedded brain tumors tissues using LMNB1 mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 9:Immunohistochemical analysis of paraffin-embedded cerebellum tissues using LMNB1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/aa623f13fa1cd17cb2ed2d5d99b8ea5e224de93445beaccdbddf5c18b94cada0.jpg?v=1666705792)
Figure 9:Immunohistochemical analysis of paraffin-embedded cerebellum tissues using LMNB1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.