LIN28 Primary Antibody
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Description
LIN28: lin-28 homolog (C. elegans), also known as CSDD1, ZCCHC1. Entrez Protein NP_078950. LIN28 was first discovered in the nematode C. elegans. It is a heterochronic protein in C. elegans involved in the timing of developmental events and choice of stage specific cell fates. LIN28 expression has been found to be regulated post-transcriptionally by miRNAs in both nematodes and mammals. In humans it is expressed in embryonic stem cells and its expression decreases during differentiation. It is negatively regulated by retinoic acid in neuronal differentiation.
Product Overview
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
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Western Blot

Figure 1: Western blot analysis using LIN28 mouse mAb against NTERA-2 cell lysate (1).
Immunofluorescence analysis

Figure 2: Confocal Immunofluorescence analysis of methanol fixed Hela cells were transfected with pMX construct of human LIN28, cells were analyzed ~62 hours after transfection.
Immunofluorescence analysis

Figure 3: Confocal Immunofluorescence analysis of NTERA-2 cells using LIN28 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
For Research Use Only. Not for use in diagnostic procedures.