LIN28 Primary Antibody

Item Information

Catalog #

Size

Price

Description

LIN28: lin-28 homolog (C. elegans), also known as CSDD1, ZCCHC1. Entrez Protein NP_078950. LIN28 was first discovered in the nematode C. elegans. It is a heterochronic protein in C. elegans involved in the timing of developmental events and choice of stage specific cell fates. LIN28 expression has been found to be regulated post-transcriptionally by miRNAs in both nematodes and mammals. In humans it is expressed in embryonic stem cells and its expression decreases during differentiation. It is negatively regulated by retinoic acid in neuronal differentiation.

Product Overview

Entrez GenelD

79727

Aliases

CSDD1; LIN-28; LIN28A; ZCCHC1

Clone#

6D1F9

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of LIN28 (aa93-209) expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Dev Dyn. 2005 Feb;232(2):487-97.
2. Mol Cell Biol. 2005 Nov;25(21):9198-208.

Product Image

Western Blot

Figure 1: Western blot analysis using LIN28 mouse mAb against NTERA-2 cell lysate (1).

Immunofluorescence analysis

Figure 2: Confocal Immunofluorescence analysis of methanol fixed Hela cells were transfected with pMX construct of human LIN28, cells were analyzed ~62 hours after transfection.

Immunofluorescence analysis