LIN28 Primary Antibody

Item Information
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Description

LIN28: lin-28 homolog (C. elegans), also known as CSDD1, ZCCHC1. Entrez Protein NP_078950. LIN28 was first discovered in the nematode C. elegans. It is a heterochronic protein in C. elegans involved in the timing of developmental events and choice of stage specific cell fates. LIN28 expression has been found to be regulated post-transcriptionally by miRNAs in both nematodes and mammals. In humans it is expressed in embryonic stem cells and its expression decreases during differentiation. It is negatively regulated by retinoic acid in neuronal differentiation.

Product Overview
Entrez GenelD
79727
Aliases
CSDD1; LIN-28; LIN28A; ZCCHC1
Clone#
6D1F9
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of LIN28 (aa93-209) expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Dev Dyn. 2005 Feb;232(2):487-97.
2. Mol Cell Biol. 2005 Nov;25(21):9198-208.
Product Image
Western Blot
Figure 1: Western blot analysis using LIN28 mouse mAb against NTERA-2 cell lysate (1).
Figure 1: Western blot analysis using LIN28 mouse mAb against NTERA-2 cell lysate (1).
Immunofluorescence analysis
Figure 2: Confocal Immunofluorescence analysis of methanol fixed Hela cells were transfected with pMX construct of human LIN28, cells were analyzed ~62 hours after transfection.
Figure 2: Confocal Immunofluorescence analysis of methanol fixed Hela cells were transfected with pMX construct of human LIN28, cells were analyzed ~62 hours after transfection.
Immunofluorescence analysis
Figure 3: Confocal Immunofluorescence analysis of NTERA-2 cells using LIN28 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Figure 3: Confocal Immunofluorescence analysis of NTERA-2 cells using LIN28 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
For Research Use Only. Not for use in diagnostic procedures.