LGR5 Primary Antibody
Item Information
Catalog #
Size
Price
Description
LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5) is a Protein Coding gene. Among its related pathways are Wnt signaling pathway (KEGG). GO annotations related to this gene include G-protein coupled receptor activity and transmembrane signaling receptor activity. An important paralog of this gene is LGR6.
Product Overview
Entrez GenelD
8549
Aliases
FEX; HG38; GPR49; GPR67; GRP49
Clone#
2B5B9
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human LGR5 (AA: 22-178) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Tumour Biol. 2014 Nov;35(11):11605-12.
2.Int J Mol Med. 2014 Jul;34(1):35-42.
2.Int J Mol Med. 2014 Jul;34(1):35-42.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using LGR5 mAb against human LGR5 (AA: 22-178) recombinant protein. (Expected MW is 43.4 kDa)
Western Blot
Figure 3:Western blot analysis using LGR5 mAb against HEK293 (1) and LGR5 (AA: 22-178)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of A549 cells using LGR5 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.