Mouse Monoclonal Antibody to KRT9
Item Information
Catalog #
Size
Price
Description
This gene encodes the type I keratin 9, an intermediate filament chain expressed only in the terminally differentiated epidermis of palms and soles. Mutations in this gene cause epidermolytic palmoplantar keratoderma.
Product Overview
Entrez GenelD
3857
Aliases
K9; CK-9; EPPK
Clone#
8C2A6
Host / Isotype
Mouse / IgG2b
Immunogen
Purified recombinant fragment of human KRT9 (AA: 153-460) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cell Physiol Biochem. 2018;46(5):1919-1929. 2.Cancer Genet. 2016 Nov;209(11):515-524.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using KRT9 mAb against human KRT9 (AA: 153-460) recombinant protein. (Expected MW is 51.7 kDa)
Western Blot
Figure 3:Western blot analysis using KRT9 mAb against HEK293-6e (1) and KRT9 (AA: 153-460)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using KRT9 mouse mAb against HepG2 (1), MCF-7 (2), NIH/3T3 (3), rat heart (4), mouse heart (5), Hela (6), and HUVEC (7) cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of A431 cells using KRT9 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using KRT9 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using KRT9 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.