Mouse Monoclonal Antibody to KRT6A

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Description

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. This KRT6 gene in particular encodes the most abundant isoform. Mutations in these genes have been associated with pachyonychia congenita. In addition, peptides from the C-terminal region of the protein have antimicrobial activity against bacterial pathogens. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Product Overview
Entrez GenelD
3853
Aliases
KRT6A; K6A; K6C; K6D; PC3; CK6C; CK6D; CK-6C; CK-6E; KRT6C; KRT6D
Clone#
7E3D1
Host / Isotype
Mouse / IgG2b
Immunogen
Purified recombinant fragment of human KRT6A (AA: 253-352) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Cell Biol. 2018 Feb 5;217(2):731-744. 2.Pediatr Dermatol. 2016 May;33(3):337-42.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using KRT6A mAb against human KRT6A (AA: 253-352) recombinant protein. (Expected MW is 37.2 kDa)
Figure 2:Western blot analysis using KRT6A mAb against human KRT6A (AA: 253-352) recombinant protein. (Expected MW is 37.2 kDa)
Western Blot
Figure 3:Western blot analysis using KRT6A mAb against HEK293-6e (1) and KRT6A (AA: 253-352)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using KRT6A mAb against HEK293-6e (1) and KRT6A (AA: 253-352)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using KRT6A mouse mAb against Hela (1), MCF-7 (2), SW480 (3), HepG2 (4), and T47D (5) cell lysate.
Figure 4:Western blot analysis using KRT6A mouse mAb against Hela (1), MCF-7 (2), SW480 (3), HepG2 (4), and T47D (5) cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of Hela cells using KRT6A mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Hela cells using KRT6A mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 6:Immunofluorescence analysis of Hela cells using KRT6A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 6:Immunofluorescence analysis of Hela cells using KRT6A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.