Mouse Monoclonal Antibody to KRT6A

Item Information

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Description

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. This KRT6 gene in particular encodes the most abundant isoform. Mutations in these genes have been associated with pachyonychia congenita. In addition, peptides from the C-terminal region of the protein have antimicrobial activity against bacterial pathogens. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Product Overview

Entrez GenelD

3853

Aliases

KRT6A; K6A; K6C; K6D; PC3; CK6C; CK6D; CK-6C; CK-6E; KRT6C; KRT6D

Clone#

7E3D1

Host / Isotype

Mouse / IgG2b

Immunogen

Purified recombinant fragment of human KRT6A (AA: 253-352) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4℃; -20℃ for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.J Cell Biol. 2018 Feb 5;217(2):731-744. 2.Pediatr Dermatol. 2016 May;33(3):337-42.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using KRT6A mAb against human KRT6A (AA: 253-352) recombinant protein. (Expected MW is 37.2 kDa)

Western Blot

Figure 3:Western blot analysis using KRT6A mAb against HEK293-6e (1) and KRT6A (AA: 253-352)-hIgGFc transfected HEK293-6e (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using KRT6A mouse mAb against Hela (1), MCF-7 (2), SW480 (3), HepG2 (4), and T47D (5) cell lysate.

Flow cytometric analysis

Figure 5:Flow cytometric analysis of Hela cells using KRT6A mouse mAb (green) and negative control (red).

Immunofluorescence analysis

Figure 6:Immunofluorescence analysis of Hela cells using KRT6A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

For Research Use Only. Not for use in diagnostic procedures.